PDGFB (PDGF-BB) Human Protein

CAT#: DA3553S

PDGFB (PDGF-BB) human recombinant protein, 2 µg

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Predicted MW: 24.3 kDa
• Purity: >95% pure by SDS-PAGE and visualised by silver stain.
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized purified protein
  Buffer System: 50 mM Acetic Acid without stabilizer
• Bioactivity: Biological: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb-c 3T3 cells is < 1 ng/ml.
  Specific: > 1 x 10e6 units/mg
• Endotoxin: < 0.1 ng per ug of PDGF-BB
• Preparation: Lyophilized purified protein
• Protein Description: Recombinant Human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
• Storage: Lyophilized Human PDGF-BB is stable for a few weeks at RT but best stored at -20°C. Reconsituted is best stored at -20°C to -70°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.

Reference Data

• RefSeq: NP_002599
• Locus ID: 5155
• UniProt ID: P01127, A0A384NYY3
• Cytogenetics: 22q13.1
• Synonyms: c-sis; IBGC5; PDGF-2; PDGF2; SIS; SSV
• Summary: This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit B, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit A. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Mutations in this gene are associated with meningioma. Reciprocal translocations between chromosomes 22 and 17, at sites where this gene and that for collagen type 1, alpha 1 are located, are associated with dermatofibrosarcoma protuberans, a rare skin tumor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]
• Protein Families: Druggable Genome
• Protein Pathways: Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma