KITLG / SCF Human Protein

CAT#: DA3548S

KITLG / SCF human recombinant protein, 2 µg

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Predicted MW: 18.5 kDa
• Purity: >98% > 98% by RP-HPLC, Anion-exchange FPLC, Silver stain
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized without stabilizer
  Buffer System: 10 mM acetic acid
• Bioactivity: Biological: Recombinant human SCF is fully biologically active when compared to standards. The ED50 determined by the dose-dependent stimulation of human TF-1 cells is < 2.0 ng/ml.
  Specific: 1 x 10e5 units/mg
• Endotoxin: < 0.1 ng per µg (IEU/µg) of rh SCF
• Reconstitution: The lyophilized powder should be reconstituted in water to a concentration not less than 0.1 mg/ml. This solution can be stored at 4°C for future use or diluted into other buffered solutions. Further dilutions should be made into buffer containing carrier protein or medium containing serum.
• Preparation: Lyophilized without stabilizer
• Protein Description: Recombinant Human SCF
• Note: Centrifuge vials before opening!
• Storage: Prior and following reconstitution store (in aliquots) at 2 - 8 °C for up to two weeks or -20 °C for longer. For long term storage add a carrier protein (0.1 % HAS or BSA).
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.

Reference Data

• RefSeq: NP_000890
• Locus ID: 4254
• UniProt ID: P21583, A0A024RBC0
• Cytogenetics: 12q21.32
• Synonyms: DCUA; DFNA69; FPH2; FPHH; Kitl; KL-1; MGF; SCF; SF; SHEP7; SLF
• Summary: This gene encodes the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer