Interleukin-3 / IL3 Human Protein

CAT#: DA3545

Interleukin-3 / IL3 human recombinant protein, 10 µg

Size: 2 ug 10 ug


USD 256.00

2 Weeks*

Size
    • 10 ug

Product Images

Specifications

Product Data
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence
MAPMTQTTPL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF
N-terminal Sequence: MAPMTQT
Predicted MW 15 kDa
Purity >98% pure by SDS-PAGE & silver stain
Presentation Purified
Buffer Presentation State: Purified
State: Lyophilized purified protein
Buffer System: PBS
Stabilizer: None
Bioactivity Biological: Recombinant human IL-3 is fully biologically active when compared to standards.
The ED50 as determined by the dose-dependent stimulation of the proliferation of TF-1 cells is < 0.1-0.5 ng/ml.
Specific: 1 x 107 units/mg
Endotoxin < 0.1 ng per µg (IEU/µg) of rh IL-3.
Reconstitution Restore in water to a concentration not less than of 0.1 mg/ml.
This solution can be diluted into other buffered solutions or stored at -20°C for future use.
For most in vitro applications, IL-3 exerts its biological activity in the concentration range of 0.1 to 1.0 ng/ml.
Preparation Lyophilized purified protein
Protein Description Recombinant Human IL-3 produced in E. coli is a 15.0 kDa globular protein containing 133 amino acid residues.
Note Centrifuge vial before opening!
Storage Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_000579
Locus ID 3562
UniProt ID P08700
Cytogenetics 5q31.1
Synonyms IL-3; MCGF; MULTI-CSF
Summary The protein encoded by this gene is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders. [provided by RefSeq, Jul 2008]
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.