GM-CSF Human Protein

CAT#: DA3544X

GM-CSF human protein, 50 µg

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Predicted MW: 14.5 kDa
• Purity: >98% > 98% by SDS-PAGE and HPLC analyses
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized without stabilizer or preservative
• Bioactivity: Biological: Human GM-CSF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is < / = 0.1 ng/ml.
  For most in vitro applications, human GM-CSF exerts its biologival activity in the concentration range of 0.05 - 0.5 ng/ml.
  Specific: 1x10e7 units/mg
• Endotoxin: < 0.1 ng per µg of GM-CSF
• Reconstitution: The lyophilized GM-CSF is soluble in water and most aqueous buffers.
  The lyophilized powder can be reconstituted in water to a concentration of 0.1 mg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.
• Preparation: Lyophilized without stabilizer or preservative
• Protein Description: Human GM-CSF is a 14,6 kDa protein consisting of 123 amino acid residues
• Storage: The lyophilized protein is stable for one year from despatch at -20°C.
  Reconstituted GM-CSF can be stored for three months in working aliquots at -20°C.
  Avoid repeated freezing and thawing.

Reference Data

• RefSeq: NP_000749
• Locus ID: 1437
• UniProt ID: P04141
• Cytogenetics: 5q31.1
• Synonyms: CSF; GMCSF
• Summary: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. This gene plays a role in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been detected in SARS-CoV-2 infected patients that develop acute respiratory distress syndrome. Mice deficient in this gene or its receptor develop pulmonary alveolar proteinosis. [provided by RefSeq, Aug 2020]
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway