Tumor necrosis factor (TNF-alpha)

CAT#: DA3542X

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Recombinant Human TNF-a produced in E. coli is a single, non-glycosylated polypeptide having a Molecular Mass of 17.4 kDa protein and consisting of 158 amino acid residues.


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USD 230.00


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2 Weeks

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    • 50 ug


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Product Data
Description Recombinant Human TNF-a produced in E. coli is a single, non-glycosylated polypeptide having a Molecular Mass of 17.4 kDa protein and consisting of 158 amino acid residues.
Species Human
Expression Host E. coli
Predicted MW 17.48 kDa
Purity > 98 % by RP-HPLC, Anion-exchange FPLC, Silver stain
Buffer 20mM PBS, pH 7.2, 10mM NaCl
Bioactivity 2 x 107 units/mg Human rh TNF-alpha is fully biologically active when compared to standards.
The ED50 as determined by the cytolysis of Murine L929 cells in the presence of Actinomycin-D is < 0.05 ng/ml.
Endotoxin < 0.1 ng per µg (IEU/µg) of rh TNF-alpha
Reconstitution Restore in sterile water to a concentration of 0.1 mg/ml.
This solution can be diluted into other buffered solutions or stored at –20°C for future use.
For most in-vitro applications, TNF-alpha exerts its biological activity in the concentration range of 0.05 to 20.0 ng/ml.
Preparation Lyophilized purified fraction
Reference Data
RefSeq NP_000585
Locus ID 7124
Cytogenetics 6p21.33
Synonyms DIF; TNF-alpha; TNFA; TNFSF2; TNLG1F
Summary This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine. [provided by RefSeq, Jul 2008]
Protein Families Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
Protein Pathways Adipocytokine signaling pathway, Allograft rejection, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Asthma, Cytokine-cytokine receptor interaction, Dilated cardiomyopathy, Fc epsilon RI signaling pathway, Graft-versus-host disease, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), MAPK signaling pathway, Natural killer cell mediated cytotoxicity, NOD-like receptor signaling pathway, RIG-I-like receptor signaling pathway, Systemic lupus erythematosus, T cell receptor signaling pathway, TGF-beta signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus, Type II diabetes mellitus

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68 Mouse Clones