VEGFR-1 / Flt-1 (D1-D5) Human Protein
VEGFR-1 / Flt-1 (D1-D5) human recombinant protein, 5 µg
|Description||VEGFR-1 / Flt-1 (D1-D5) human recombinant protein, 5 µg|
|Expression cDNA Clone or AA Sequence||SKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKESERLSITKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESAIYIFISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVTLKKFPLDTLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATVNGHLYKTNYLTHRQTNTIIDVQISTPRPVKLLRGHTLVLNCTATTPLNTRVQMTWSYPDEKNKRASVRRRIDQSNSHANIFYSVLTIDKMQNKDKGLYTCRVRSGPSFKSVNTSVHIYDKAFITVKHRKQQVLETVAGKRSYRLSMKVKAFPSPEVVWLKDGLPATEKSARYLTRGYSLIIKDVTEEDAGNYTILLSIKQSNVFKNLTATLIVNVKPQIYEKAVSSFPDPALYPLGSRQILTCTAYGIPQPTIKWFWHPCNHNHSEARCDFCSNNEESFILDADSNMGNRIESITQRMAIIEGKNKMASTLVVADSRISGIYICIASNKVGTVGRNISFYITDVPNGFHVN|
|Predicted MW||70 kDa|
|Purity||>90% pure by SDS-PAGE and Silver stain|
|Buffer||Presentation State: Purified
State: Lyophilized purified protein
Buffer System: PBS
|Bioactivity||Biological: The activity of recombinant Human sVEGFR-1D1-5 was determined by its ability to to inhibit the VEGF-A-induced proliferation of HUVECs.|
|Endotoxin||< 0.1 ng per mg of VEGFR-1|
|Reconstitution||The lyophilized sVEGFR-1D1-5 is soluble in water and most aqueous buffers and should be restored in PBS to a concentration not lower than 100 ng/ml.|
|Preparation||Lyophilized purified protein|
|Protein Description||Recombinat Human soluble Vascular Endothelial Growth Factor Receptor-1 domain D1-5 (sVEGFR-1D1-5) is a 72 kDa protein containing amino acid residues. The baculovirus generated, recombinant Human sVEGFR-1 is produced as a non-chimeric protein in a monomeric form. The soluble receptor protein contains only the first 5 extracellular domains, which contain all the information necessary for high affinity ligand binding. The receptor monomers have a mass of approximately 70kDa.
Result by N-terminal sequencing: SGSKLKD
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Synonyms||FLT; FLT-1; VEGFR-1; VEGFR1|
|Summary||This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.[provided by RefSeq, May 2009]|
|Protein Families||Druggable Genome, Protein Kinase, Secreted Protein|
|Protein Pathways||Cytokine-cytokine receptor interaction, Endocytosis, Focal adhesion|