VEGF-C / Flt4-L (VEGF-C152S) Rat Protein

CAT#: DA3520

VEGF-C / Flt4-L (VEGF-C152S) rat recombinant protein, 5 µg

Specifications

Product Data

• Species: Rat
• Expression Host: Insect
• Predicted MW: 18-24 kDa
• Purity: >90% by SDS-PAGE and visualised by silver stain
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized protein
  Buffer System: 50 mM Acetic Acid
  Stabilizer: BSA
• Bioactivity: Biological: Measured by its ability to stimulate phosphorylation of the VEGFR-3/FLT-4 receptor in porcine aortic endothelial cells (PAE/FLT-4 cells). The ED50 for this effect is typically 150-300 ng/ml.
• Endotoxin: < 0.1 ng per µg of VEGF-C152S
• Reconstitution: The lyophilized VEGF-C152S is soluble in water and most aqueous buffers. Restore in PBS or medium to a concentration not lower than 50 µg/ml.
• Preparation: Lyophilized protein
• Protein Description: VEGF-C152S is a point mutant generated by the replacement of the second conserved Cys residue of the recombinant processed VEGF-C by a Ser residue. VEGF-C152S is analog to the human VEGF-C156S mutant and only active toward VEGFR-3/FLT-4 but, unlike wild type VEGF-C, is unable to bind to and to activate signalling through VEGFR-2/KDR. VEGF-C152S was inactive in the vascular permeability assay and did not increase migration of the capillary endothelial cells, indicating that these VEGF-like effects of VEGF-C require VEGFR-2 binding.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_446105
• Locus ID: 114111
• UniProt ID: O35757
• Cytogenetics: 16p11
• Summary: platelet-derived growth factor/vascular derived growth factor (PDGF/VEGF); active in angiogenesis and endothelial cell growth [RGD, Feb 2006]