VEGF-E

CAT#: DA3516

Recombinant ov-VEGF-E (Orf virus). A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa.


 Product Datasheet for 'DA3516'

USD 260.00


Availability*
2 Weeks

Size
    • 5 ug


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Specifications

Product Data
Description Recombinant ov-VEGF-E (Orf virus). A DNA sequence encoding the mature variant of ovVEGF-E isolate D1701 (Dehio et al., 1999; GenBank accession No. AF106020) was expressed in E. coli as a 132 amino acid residue fusion protein with an N-terminal His-tag sequence and a thrombin cleavage site. Recombinant VEGF-E homodimer was dimerized in vitro and has a predicted mass of approximately 35 kDa.
Species Orf Virus
Expression Host E.Coli
Tag Tag Free
Predicted MW 35 kDa
Purity > 90 % pure by SDS-PAGE and visualised by silver stain.
Buffer PBS, pH 7.4 without stabilizers.
Bioactivity 2 x 10e5 units/mg The ED50 for stimulation of 3H-thymidine incorporation and cell proliferation by human umbilical vein endothelial cells for VEGF-E has been determined to be in the range of 5-20 ng/ml.
Endotoxin < 0.1 ng per µg of VEGF-E
Reconstitution Restore in PBS or medium containing at least 0.1% human or bovine serum albumin to a concentration not lower than 50 µg/ml.
(VEGF-E is soluble in water and most aqueous buffers).
Preparation Lyophilized
Reference Data
RefSeq

Documents

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