GM-CSF (His-tag) Human Protein

CAT#: DA3505XC

GM-CSF (His-tag) human recombinant protein, 20 µg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: Insect
• Tag: His-tag
• Predicted MW: 14.6 kDa
• Purity: >98% pure by SDS-PAGE and visualised by silver stain.
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized without preservatives and stabilizers
  Buffer System: PBS
• Bioactivity: Biological: Human GM-CSF is fully biologically active when compared to standards.
  Measured in a cell proliferation assay using TF-1 human erythroleukemic cells [Kitamura T et al, J Cell Physiol, 1989]. The ED50 for this effect is typically < 0.1ng/ml corresponding to a specific activity of ≥ 1 x 10⁷ units/mg.
• Endotoxin: < 0.1 ng per µg of GM-CSF
• Reconstitution: The lyophilized GM-CSF is soluble in water and most aqueous buffers.
  The lyophilized powder can be reconstituted in water to a concentration of 0.1 mg/ml. This solution can be diluted into other buffered solutions or stored at -20°C for future use.
• Preparation: Lyophilized without preservatives and stabilizers
• Protein Description: Recombinant human GM-CSF.
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_000749
• Locus ID: 1437
• UniProt ID: P04141
• Cytogenetics: 5q31.1
• Synonyms: CSF; GMCSF
• Summary: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. This gene plays a role in promoting tissue inflammation. Elevated levels of cytokines, including the one produced by this gene, have been detected in SARS-CoV-2 infected patients that develop acute respiratory distress syndrome. Mice deficient in this gene or its receptor develop pulmonary alveolar proteinosis. [provided by RefSeq, Aug 2020]
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
• Protein Pathways: Cytokine-cytokine receptor interaction, Fc epsilon RI signaling pathway, Hematopoietic cell lineage, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway