Hepatitis B Surface Antigen / HBsAg (ayw) Protein

CAT#: BIN061

Hepatitis B Surface Antigen / HBsAg (ayw) recombinant protein, 0.5 mg

Specifications

Product Data

• Expression Host: S. cerevisiae
• Concentration: lot specific
• Purity: >98% pure by SDS-PAGE).
  Purification is conducted from broken yeast cells by the following scheme: Clarification, Microfiltration, Ultrafiltration, Adsorption Chromatography, Ion-exchange chromatography, Ultracentrifugation and Sterile Filtration. Product is sterile filtered.
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid (sterile filtered) purified protein.
  Buffer System: 0.05M Phosphate, 0.2M NaCl, pH 7.2 without preservatives.
• Preparation: Liquid (sterile filtered) purified protein.
• Applications: Tested in ELISA with anti-HBsAg antibodies.
• Protein Description: Recombinant Hepatitis B Surface Antigen (HBsAg), (ayw) (S region).
  Recombinant HBsAg is a 24 kDa protein.
  Genotype B.
  Does not contain a fusion partner.
• Storage: Store the antigen at 2-8°C.
• Stability: Shelf life: six months from despatch.

Reference Data

• Summary: Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.