CKMB Type 1 Protein

CAT#: BIN012

CKMB Type 1 recombinant protein, 1 mg

Specifications

Product Data

• Expression Host: Pichia pastoris
• Concentration: lot specific
• Purity: >95% pure by SDS-PAGE. Purified under non-denaturing conditions.
• Presentation: Azide Free
• Buffer: Presentation State: Azide Free
  State: Liquid purified protein
  Buffer System: 0.02M Potassium phosphate, 1 mM DTT, 50% Glycerol, pH 5.0-6.0
  Preservative: None
• Preparation: Liquid purified protein
• Applications: ELISA.
  Western blot.
• Protein Description: Recombinant CK-MB Type 1.
  Recombinant Creatine Kinase MB (CK-MB) Type 1.
  Recombinant full length Creatine Kinase MB isoenzyme without the C-terminal lysine on the M subunit.
  CK-MB is a dimeric protein comprised of M and B subunits. The doublet band migrating approximately 44 kDa is not resolved on most gels. Purified in the enzymatically active form.
  Reacts with monoclonal antibodies specific to MB isoenzyme in ELISA.
• Storage: Upon receipt, store (in aliquots) at -20°C to -80°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: Creatine phosphokinase, also known as creatine kinase (CK), is an enzyme expressed by various tissues and cell types. CK catalyses the conversion of creatine and consumes adenosine triphosphate (ATP) to create phosphocreatine and adenosine diphosphate (ADP). In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB.