Neurofilament M (160 kD) Bovine Protein

CAT#: BA1013

Neurofilament M (160 kD) bovine protein, 0.25 mg

Product Images

Lane 1 shows myosin (a), beta-galactosidase (b), phosphorylase B (c), BSA (d) and ovalbumin (e) as markers Lane 2 shows Cat.No. BA1014/BA1014S Neurofilament H (200 kD) Lane 3 shows Cat.No. BA1013/BA1013S Neurofilament M (160 kD) Lane 4 shows Cat.No. BA1012/BA1012S Neurofilament L (68kDa)

Specifications

Product Data

• Species: Bovine
• Protein Source: Spinal Cord
• Predicted MW: 160 kDa
• Concentration: lot specific
• Purity: >98% (determined by SDS gelelectrophoresis)
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized
• Reconstitution: BA1013: Restore with 200 µl distilled water (final volume 250 μl).
  BA1013S: Restore with 80 µl distilled water (final volume 100 µl).
  Final solution: 10mM Sodium Phosphate, pH 7.5, 2mM DTT, 6M Urea, 1 mM EDTA.
• Preparation: Lyophilized
• Applications: Protein standard in 1D and 2D SDS gelelectrophoresis.
  Immunoassays.
  Immunization.
• Protein Description: Bovine Neurofilament 160 kDa
• Note: Isoelectric Point: pI 5.1
• Storage: Store at 2-8°C (lyophilized) and at -20°C (reconstituted).
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001099011
• Locus ID: 4741
• Cytogenetics: 8p21.2
• Synonyms: NEF3; NF-M; NFM
• Summary: Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)