Filamin Chicken Protein

CAT#: BA1010

Filamin chicken protein, 0.25 mg

Product Images

Specifications

Product Data

• Species: Chicken
• Protein Source: Gizzard
• Predicted MW: 250 kDa
• Concentration: lot specific
• Purity: >90% > 90% (determined by SDS gelelectrophoresis)
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Lyophilized
  Buffer System: 20 mM Tris/acetate buffer pH 7.6, 0.1 mM EDTA, 2 mM DTT, 20 mM NaCl
• Reconstitution: Restore with 250 µl Laemmli buffer (final volume 250 µl) or 9M urea buffer (e.g. IEF or NEPHGE buffer) Sometimes filamin is not readily soluble after lyophilization. In this case add 250 µl dist. water, after dissolution add 135 mg solid urea (to reach an end concentration of 9 M urea), leave for 1h at room temperature, separate insoluble rests by a short centrifugation step.
• Preparation: Lyophilized
• Applications: Protein standard in 1D and 2D SDS gelelectrophoresis.
  Immunoassays.
  Immunization.
• Protein Description: Filamin, purified polypeptide
• Note: Isoelectric Point: pI 6.4
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the protein undiluted at -70°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.