Maltose Binding Protein Tag / MBP-Tag (27-396) Escherichia coli Protein

CAT#: AR52073PU-N

Maltose Binding Protein Tag / MBP-Tag (27-396) e. coli protein, 0.5 mg

Product Images

Specifications

Product Data

• Species: Escherichia coli
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTRIT K
• Predicted MW: 40.8 kDa
• Concentration: lot specific
• Purity: >95% by SDS - PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: Phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Preparation: Liquid purified protein
• Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: MBP also as known as Maltose Binding Protein is a protein related with the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has been used to increase the yield of its fusion partner in many cases. In addition, MBP is often able to promote the solubility of polypeptides to which it is fused. Recombint MBP was expressed in E.coli and purified by conventiol chromatography techniques.