LGALS3 (1-264, His-tag) Mouse Protein

CAT#: AR52003PU-N

LGALS3 (1-264, His-tag) mouse protein, 50 µg

Product Images

Specifications

Product Data

• Species: Mouse
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MGSMADSFSL NDALAGSGNP NPQGYPGAWG NQPGAGGYPG AAYPGAYPGQ APPGAYPGQA PPGAYPGQAP PSAYPGPTAP GAYPGPTAPG AYPGSTAPGA FPGQPGAPGA YPSAPGGYPA AGPYGVPAGP LTVPYDLPLP GGVMPRMLIT IMGTVKPNAN RIVLDFRRGN DVAFHFNPRF NENNRRVIVC NTKQDNNWGK EERQSAFPFE SGKPFKIQVL VEADHFKVAV NDAHLLQYNH RMKNLREISQ LGISGDITLT SANHAMI
• Tag: His-tag
• Predicted MW: 29.8 kDa
• Concentration: lot specific
• Purity: >95% by SDS - PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 50% glycerol, 1 mM DTT, 2 mM EDTA
• Bioactivity: Specific: The ED50 for this effect is equal or higher than 25 ug/ml. Measured by its ability to agglutinate human red blood cells.
• Preparation: Liquid purified protein
• Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001139425
• Locus ID: 16854
• UniProt ID: Q8C253
• Cytogenetics: 14 C1
• Synonyms: gal3; GBP; L-34; Mac-2
• Summary: LGALS3, also known as galectin 3, is a member of the family of animal lectins, which selectively binds beta-galactoside residues. This protein is secreted from cells by ectocytosis, which is independent of the classical secretory pathway through the endoplasmic reticulum/Golgi network. LGALS3 has been associated with the inhibition of apoptosis and the progression of cancer. It is normally distributed in epithelia of many organs, in various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. Recombint mouse LGALS3 protein, used to His-tag at N-terminus, was expressed in E.coli and purified by using conventiol chromatography techniques.