Erythropoietin / EPO (28-193, His-tag)
Specifications
Product Data | |
Species | Human |
Expression Host | Insect |
Tag | His-tag |
Predicted MW | 19.5 kDa |
Concentration | 0.5 mg/ml (determined by absorbance at 280nm) |
Purity | > 95 % by SDS - PAGE |
Buffer | Phosphate Buffered Saline (pH 7.4) containing 10% glycerol. |
Bioactivity | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is equal or less than0.5 ng/ml. |
Endotoxin | < 1.0 EU per 1 microgram of protein (determined by LAL method) |
Preparation | Liquid purified protein |
Reference Data | |
RefSeq | NP_000790 |
Locus ID | 2056 |
Cytogenetics | 7q22.1 |
Synonyms | DBAL; ECYT5; EP; MVCD2 |
Summary | This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017] |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane |
Protein Pathways | Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway |
Documents
FAQs |
SDS |
Resources
Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LY400277 | Transient overexpression lysate of erythropoietin (EPO) |
USD 315.00 |
|
AR31186PU-N | Human EPO Cat.-No AR31186PU contains 166 amino acid residues and has a calculated molecular weight of approximately 18.4 kDa. As a result of glycosylation, Recombinant Human EPO migrates with an apparent molecular mass of 37.0 kDa by SDS-PAGE gel, under reducing and non-reducing conditions. |
USD 250.00 |
|
TP723837 | Purified recombinant protein of Human erythropoietin (EPO) |
USD 255.00 |
|
AR51115PU-S | Recombinant human EPO protein, fused to His-tag at N-terminus, was expressed in E.coli. |
USD 400.00 |
|
AR05112PU-N | This antigen is Recombinant Human Erythropoietein alpha, produced in CHO cells. It is a single polypeptide chain containing 166 amino acids with a predicted molecular mass of 21,000 Daltons and an apparent molecular mass of 30,400 Daltons. |
USD 460.00 |
|
LC400277 | EPO HEK293T cell transient overexpression lysate (as WB positive control) |
USD 95.00 |
|
AR51115PU-N | Recombinant human EPO protein, fused to His-tag at N-terminus, was expressed in E.coli. |
USD 1,070.00 |
|
AR31186PU-S | Human EPO Cat.-No AR31186PU contains 166 amino acid residues and has a calculated molecular weight of approximately 18.4 kDa. As a result of glycosylation, Recombinant Human EPO migrates with an apparent molecular mass of 37.0 kDa by SDS-PAGE gel, under reducing and non-reducing conditions. |
USD 140.00 |
|
AR51963PU-N |
USD 1,070.00 |