Erythropoietin / EPO (28-193, His-tag) Human Protein

CAT#: AR51963PU-S

Product images

Specifications

Product Data

• Species: Human
• Expression Host: Insect
• Expression cDNA Clone or AA Sequence: APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALRAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDRLEHH HHHH
• Tag: His-tag
• Predicted MW: 19.5 kDa
• Concentration: 0.5 mg/ml (determined by absorbance at 280nm)
• Purity: > 95 % by SDS - PAGE
• Buffer: Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Bioactivity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is equal or less than0.5 ng/ml.
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation: Liquid purified protein

Reference Data

• RefSeq: NP_000790
• Locus ID: 2056
• Cytogenetics: 7q22.1
• Synonyms: DBAL; ECYT5; EP; MVCD2
• Summary: This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway