Sonic hedgehog (SHH) (24-197,)

CAT#: AR51857PU-N

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Recombinant human SHH was expressed in E.coli and purified by using conventional chromatography techniques.

 Product Datasheet for 'AR51857PU-N'

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USD 845.00

2 Weeks

    • 250 ug

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Product Data
Description Recombinant human SHH was expressed in E.coli and purified by using conventional chromatography techniques.
Species Human
Expression Host E. coli
Predicted MW 19.8 kDa
Concentration 1.0 mg/ml (determined by Bradford assay)
Purity > 95 % by SDS - PAGE
Buffer Phosphate buffered saline (pH7.4).
Endotoxin < 1.0 Eu per 1 microgram of protein (determined by LAL method)
Preparation Liquid purified protein
Reference Data
RefSeq NP_000184
Locus ID 6469
Cytogenetics 7q36.3
Summary This gene encodes a protein that is instrumental in patterning the early embryo. It has been implicated as the key inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Of three human proteins showing sequence and functional similarity to the sonic hedgehog protein of Drosophila, this protein is the most similar. The protein is made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the developing embryo. Defects in this protein or in its signalling pathway are a cause of holoprosencephaly (HPE), a disorder in which the developing forebrain fails to correctly separate into right and left hemispheres. HPE is manifested by facial deformities. It is also thought that mutations in this gene or in its signalling pathway may be responsible for VACTERL syndrome, which is characterized by vertebral defects, anal atresia, tracheoesophageal fistula with esophageal atresia, radial and renal dysplasia, cardiac anomalies, and limb abnormalities. Additionally, mutations in a long range enhancer located approximately 1 megabase upstream of this gene disrupt limb patterning and can result in preaxial polydactyly. [provided by RefSeq, Jul 2008]
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
Protein Pathways Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer


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68 Mouse Clones