CYP2E1 (29-493, His-tag) Human Protein
CYP2E1 (29-493, His-tag) human recombinant protein, 0.5 mg
|Description||CYP2E1 (29-493, His-tag) human recombinant protein, 0.5 mg|
|Expression Host||E. coli|
|Expression cDNA Clone or AA Sequence||MGSSHHHHHH SSGLVPRGSH MGSSWNLPPG PFPLPIIGNL FQLELKNIPK SFTRLAQRFG PVFTLYVGSQ RMVVMHGYKA VKEALLDYKD EFSGRGDLPA FHAHRDRGII FNNGPTWKDI RRFSLTTLRN YGMGKQGNES RIQREAHFLL EALRKTQGQP FDPTFLIGCA PCNVIADILF RKHFDYNDEK FLRLMYLFNE NFHLLSTPWL QLYNNFPSFL HYLPGSHRKV IKNVAEVKEY VSERVKEHHQ SLDPNCPRDL TDCLLVEMEK EKHSAERLYT MDGITVTVAD LFFAGTETTS TTLRYGLLIL MKYPEIEEKL HEEIDRVIGP SRIPAIKDRQ EMPYMDAVVH EIQRFITLVP SNLPHEATRD TIFRGYLIPK GTVVVPTLDS VLYDNQEFPD PEKFKPEHFL NENGKFKYSD YFKPFSTGKR VCAGEGLARM ELFLLLCAIL QHFNLKPLVD PKDIDLSPIH IGFGCIPPRY KLCVIPRS|
|Predicted MW||56.2 kDa|
|Concentration||0.5 mg/ml (determined by Bradford assay)|
|Purity||>80% by SDS - PAGE|
|Buffer||Presentation State: Purified
State: Liquid purified protein
Buffer System: Liquid, In 20mM Tris-HCl (pH8.0) containing 10% glycerol.
|Preparation||Liquid purified protein|
|Protein Description||Recombinant human CYP2E1, fused to His-tag at N-terminus, was expressed in E.coli.|
|Storage||Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Synonyms||CPE1; CYP2E; P450-J; P450C2E|
|Summary||This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer. [provided by RefSeq, Jul 2008]|
|Protein Families||Druggable Genome, P450, Transmembrane|
|Protein Pathways||Arachidonic acid metabolism, Drug metabolism - cytochrome P450, Linoleic acid metabolism, Metabolic pathways, Metabolism of xenobiotics by cytochrome P450|