Interleukin-2 / IL2 (21-169, His-tag) Human Protein
Interleukin-2 / IL2 (21-169, His-tag) human recombinant protein, 0.25 mg
|Description||Interleukin-2 / IL2 (21-169, His-tag) human recombinant protein, 0.25 mg|
|Expression Host||E. coli|
|Expression cDNA Clone or AA Sequence||MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF SQSIISTSPQ|
|Predicted MW||17.3 kDa|
|Concentration||0.5 mg/ml (determined by Bradford assay)|
|Purity||>90% by SDS - PAGE|
|Buffer||Presentation State: Purified
State: Liquid purified protein
Buffer System: Phosphate Buffered Saline (pH7.4).
|Bioactivity||Specific: Measured in a cell proliferation assay using CTLL-2 mouse T lymphocyte. The ED50 for this effect is < 0.9 ng/ml.|
|Endotoxin||< 1.0 Eu per 1 microgram of protein (determined by LAL method)|
|Preparation||Liquid purified protein|
|Protein Description||Recombinant mouse IL2 was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.|
|Storage||Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Cytogenetics||3 18.3 cM|
|Summary||Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. [UniProtKB/Swiss-Prot Function]|