Inositol monophosphatase 3 / IMPA3 (34-359, His-tag) Human Protein

CAT#: AR51806PU-N

Inositol monophosphatase 3 / IMPA3 (34-359, His-tag) human recombinant protein, 50 µg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MGSGRFSLFG LGGEPGGGAA GPAAAADGGT VDLREMLAVS VLAAVRGGDE VRRVRESNVL HEKSKGKTRE GAEDKMTSGD VLSNRKMFYL LKTAFPSVQI NTEEHVDAAD QEVILWDHKI PEDILKEVTT PKEVPAESVT VWIDPLDATQ EYTEDLRKYV TTMVCVAVNG KPMLGVIHKP FSEYTAWAMV DGGSNVKARS SYNEKTPRIV VSRSHSGMVK QVALQTFGNQ TTIIPAGGAG YKVLALLDVP DKSQEKADLY IHVTYIKKWD ICAGNAILKA LGGHMTTLSG EEISYTGSDG IEGGLLASIR MNHQALVRKL PDLEKTGHK
• Tag: His-tag
• Predicted MW: 37.6 kDa
• Concentration: lot specific
• Purity: >90% by SDS - PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: Phosphate Buffered Saline (pH 7.4)
• Bioactivity: Specific: Specific acitivty > 3300 pmole/min/ug, its ability to dephosphorylate adenosine 3’5’-diphosphate sodium slat at pH 7.5, 25C.
• Preparation: Liquid purified protein
• Applications: Protocol: 1. Prepare 1mM PAP in 20mM Tris-HCl (PH 7.5), 15mM MgCl2. 2. Dilute IMPAD1 various concentration (20ug/ml, 10ug/ml, 5ug/ml) in 20mM Tris-HCl (PH 7.5), 15mM MgCl2, containing blank of 20mM Tris-HCl, 15mM MgCl2 3. Make the Phosphate standard range of 0.31 nmol to 20 nmol per well. 4. Load 25 ul of 1mM PAP add 25 ul of diluted IMPAD1 to well and load 50 ul of phosphate standard. 5. Incubate for15 minute at 25C. 6. Add 150 ul of Malachite green solution mix and incubate for 15 minute at 25C. 7. Read at 650nm. - Malachite green solution: Ammonium molybdate 675 mg, Malachite green carbinol hydrochloride 3.3 mg in 50 ml of 1M H2SO4.
• Protein Description: Recombinant human IMPAD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
• Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_060283
• Locus ID: 54928
• UniProt ID: Q9NX62, A0A024R7W0
• Cytogenetics: 8q12.1
• Synonyms: GPAPP; IMP-3; IMP 3; IMPA3; IMPAD1
• Summary: This gene encodes a member of the inositol monophosphatase family. The encoded protein is localized to the Golgi apparatus and catalyzes the hydrolysis of phosphoadenosine phosphate (PAP) to adenosine monophosphate (AMP). Mutations in this gene are a cause of GRAPP type chondrodysplasia with joint dislocations, and a pseudogene of this gene is located on the long arm of chromosome 1. [provided by RefSeq, Dec 2011]
• Protein Families: Transmembrane