SIRT1 (254-495, His-tag) Human Protein

SKU AR51445PU-S

SIRT1 (254-495, His-tag) human recombinant protein, 0.1 mg

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMKK IIVLTGAGVS VSCGIPDFRS RDGIYARLAV DFPDLPDPQA MFDIEYFRKD PRPFFKFAKE IYPGQFQPSL CHKFIALSDK EGKLLRNYTQ NIDTLEQVAG IQRIIQCHGS FATASCLICK YKVDCEAVRG DIFNQVVPRC PRCPADEPLA IMKPEIVFFG ENLPEQFHRA MKYDKDEVDL LIVIGSSLKV RPVALIPSSI PHEVPQILIN REPLPHLHFD VELLGDCDVI INELCHRLGG
• Tag: His-tag
• Predicted MW: 31.6 kDa
• Concentration: lot specific
• Purity: >90% by SDS - PAGE
• Presentation State: Purified
• Buffer: Presentation State: This purified protein is available in a denatured form, making it less suitable for functional studies. Denatured proteins are better suited for applications like Western Blot (WB) or imaging assays.; State: Liquid purified protein; Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.4M Urea
• Preparation: Liquid purified protein
• Protein Description: Recombinant human SIRT1 protein, fused to His-tag at N-terminus, was expressed in E.coli .
• Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Shipping: Blue Ice

Reference Data

• RefSeq: NP_001135970
• Locus ID: 23411
• UniProt ID: Q96EB6
• Cytogenetics: 10q21.3
• Synonyms: SIRT-1, SIR2-like protein 1, SIR2L1, sirtuin type 1, sirtuin 1, sirtuin-1, SIR2alpha
• Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2008]
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors