ACADSB (34-432, His-tag)
Recombinant human ACADSB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Product Datasheet for 'AR50452PU-N'
|Description||Recombinant human ACADSB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Expression Host||E. coli|
|Predicted MW||46.4 kDa|
|Concentration||0.5 mg/ml (determined by Bradford assay)|
|Purity||> 90 % by SDS - PAGE|
|Buffer||20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT|
|Preparation||Liquid purified protein|
|Synonyms||2-MEBCAD; ACAD7; SBCAD|
|Summary||Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa. [provided by RefSeq, Jul 2008]|
|Protein Pathways||Fatty acid metabolism, Metabolic pathways, Valine, leucine and isoleucine degradation|
|LY419848||Transient overexpression lysate of acyl-Coenzyme A dehydrogenase, short/branched chain (ACADSB), nuclear gene encoding mitochondrial protein||
|LC419848||ACADSB HEK293T cell transient overexpression lysate (as WB positive control)||
|AR50452PU-S||Recombinant human ACADSB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.||