mdh (1-312, His-tag) Escherichia coli Protein

CAT#: AR50312PU-N

mdh (1-312, His-tag) recombinant protein, 0.5 mg

Product Images

Specifications

Product Data

• Species: Escherichia coli
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MGSHMKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK
• Tag: His-tag
• Predicted MW: 34.9 kDa
• Concentration: lot specific
• Purity: >95% by SDS - PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol, 50 mM NaCl
• Bioactivity: Specific: > 450 units/mg. Defined as the amount of enzyme that cleaves 1 umole of oxalacetate and beta - NADH to L - malate and beta - NAD per minute at pH 7.5 at 25°C.
  Activity Assay :
  1. Prepare a 1.45 ml assay bu ffer . The final concentrations are 100 mM Potassium phosphate , 0.13mM beta - nicotinamide adenine dinucleotide, reduced form, 0.25mM oxalacetic acid.
  2. Add 50 µl of recombinant MDH protein with various concentrat ions (0. 015 µg, 0. 03 µg ) in assay buffer.
  3. Mix by inversion and record the decrease at A340nm for approximately 5 minutes
• Preparation: Liquid purified protein
• Protein Description: Recombinant E.coli mdh protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
  Avoid repeated freezing and thawing.
  
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction). Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules.