gor (1-450, His-tag) Escherichia coli Protein

CAT#: AR50248PU-S

gor (1-450, His-tag) recombinant protein, 0.1 mg

Size: 100 ug 500 ug


USD 420.00

3 Weeks*

Size
    • 100 ug

Product Images

Specifications

Product Data
Species Escherichia coli
Expression Host E. coli
Expression cDNA Clone or AA Sequence
MGSSHHHHHH SSGLVPRGSH MGSMTKHYDY IAIGGGSGGI ASINRAAMYG QKCALIEAKE LGGTCVNVGC VPKKVMWHAA QIREAIHMYG PDYGFDTTIN KFNWETLIAS RTAYIDRIHT SYENVLGKNN VDVIKGFARF VDAKTLEVNG ETITADHILI ATGGRPSHPD IPGVEYGIDS DGFFALPALP ERVAVVGAGY IAVELAGVIN GLGAKTHLFV RKHAPLRSFD PMISETLVEV MNAEGPQLHT NAIPKAVVKN TDGSLTLELE DGRSETVDCL IWAIGREPAN DNINLEAAGV KTNEKGYIVV DKYQNTNIEG IYAVGDNTGA VELTPVAVAA GRRLSERLFN NKPDEHLDYS NIPTVVFSHP PIGTVGLTEP QAREQYGDDQ VKVYKSSFTA MYTAVTTHRQ PCRMKLVCVG SEEKIVGIHG IGFGMDEMLQ GFAVALKMGA TKKDFDNTVA IHPTAAEEFV TMR
Tag His-tag
Predicted MW 51.2 kDa
Concentration lot specific
Purity >90% by SDS - PAGE
Presentation Purified
Buffer Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.1M NaCl, 1 mM DTT
Bioactivity Specific: > 52 unit/ml.
One unit will reduce 1.0 umol of oxidized glutathione per minute at pH 7.5 at 25°C.
Preparation Liquid purified protein
Applications Protocol: 1. Prepare a 1,450 ul assay buffer. The final concentrations are 75mM Potassium phosphate (pH 7.5), 2.6mM EDTA, 1mM glutathione, 0.09mM beta-NADPH, and 0.13% BSA.
2. Add 50 ul of recombinant gor protein with 0.037 ug, 0.075 ug and 0.15 ug in assay buffer
3. Mix and load 200 ul of reaction mix in to a plate well.
4. Record the decrease in A340nm for 5 minutes at 25°C.
Protein Description Recombinant E. coli gor protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
Summary gor, also known as Glutathione reductase, belongs to the class-I pyridine nucleotide disulfide oxidoreductase family. The main function of the protein is to maintain high levels of reduced glutathione in the cytosol. With the concomitant oxidation of NADPH, Glutathione reductase transforms oxidized glutathione to the reduced form. The active site of the protein is a redox-active disulfide bond.

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.