LAT (28-233, His-tag) Human Protein

CAT#: AR50063PU-N

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LAT (28-233, His-tag) human recombinant protein, 0.25 mg


Product Datasheet for 'AR50063PU-N'


USD 845.00


Availability*
2 Weeks

Size
    • 250 ug


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Specifications

Product Data
Description LAT (28-233, His-tag) human recombinant protein, 0.25 mg
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence MGSSHHHHHH SSGLVPRGSH MHCHRLPGSY DSTSSDSLYP RGIQFKRPHT VAPWPPAYPP VTSYPPLSQP DLLPIPRSPQ PLGGSHRTPS SRRDSDGANS VASYENEEPA CEDADEDEDD YHNPGYLVVL PDSTPATSTA APSAPALSTP GIRDSAFSME SIDDYVNVPE SGESAEASLD GSREYVNVSQ ELHPGAAKTE PAALSSQEAE EVEEEGAPDY ENLQELN
Tag His-tag
Predicted MW 24.4 kDa
Concentration 1 mg/ml (determined by Bradford)
Purity >95% by SDS - PAGE
Buffer Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris-HCl buffer(pH 8.0) containing 1mM DTT
Bioactivity Specific: 1. Prepare a 200ul reaction mix into a suitable container: The final concentrations are 10mM Tris (pH7.5), 50mM NaCl, 2mM DTT, 1mM MnCl2, 10mM pNPP.
2. Pre-warm reaction mix in heat block at 37C.
3. Add recombinant DUSP3 protein solution with various concentrations (1ug, 2ug, 3ug) in 200ul reaction buffer.
4. Mix by inversion and incubate at 37C for 10minutes.
5. Stop the reaction by adding the 800ul of 0.1N NaOH and record the absorbance at 405nm.
Preparation Liquid purified protein
Protein Description Recombinant human LAT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Store undiluted at 2-8°C for one week or (in aliquots) at -20°C to -80°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_001014987
Locus ID 27040
Cytogenetics 16p11.2
Synonyms IMD52; LAT1; pp36
Summary The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
Protein Families Druggable Genome, Transmembrane
Protein Pathways Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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