G-CSF (31-204)

CAT#: AR50002PU-N

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Recombinant Human G-CSF was expressed in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.


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USD 820.00


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2 Weeks

Size
    • 250 ug


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Specifications

Product Data
Description Recombinant Human G-CSF was expressed in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Species Human
Expression Host E. coli
Predicted MW 18.8 kDa
Concentration 0.5 mg/ml (determined by Bradford assay)
Purity > 95 % by SDS - PAGE
Buffer Phosphate -buffered Saline (PBS), pH7.4, 10% Glycerol
Bioactivity Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is less than or equal to 0.3 ng/ml.
Endotoxin < 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation Liquid purified protein
Reference Data
RefSeq NP_000750
Locus ID 1440
Cytogenetics 17q21.1
Synonyms C17orf33; CSF3OS; GCSF
Summary The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway

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