G-CSF (31-204) Human Protein

CAT#: AR50002PU-N

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Recombinant Human G-CSF was expressed in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.


Product Datasheet for 'AR50002PU-N'


USD 845.00


Availability*
2 Weeks

Size
    • 250 ug


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Specifications

Product Data
Description Recombinant Human G-CSF was expressed in E.coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP
Predicted MW 18.8 kDa
Concentration 0.5 mg/ml (determined by Bradford assay)
Purity > 95 % by SDS - PAGE
Buffer Phosphate -buffered Saline (PBS), pH7.4, 10% Glycerol
Bioactivity Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 range ≤ 0.3 ng/ml.
Endotoxin < 1.0 EU per 1 microgram of protein (determined by LAL method)
Preparation Liquid purified protein
Reference Data
RefSeq NP_000750
Locus ID 1440
Cytogenetics 17q21.1
Synonyms C17orf33; CSF3OS; GCSF
Summary The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Jak-STAT signaling pathway

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