Recombinant Human BMP-7 is a 28.8 kDa homodimeric glycoprotein consisting of two 116 amino acid subunits, which correspond to amino acid residues 316 to 431 of the full-length BMP-7 precursor.
Product Datasheet for 'AR31155PU-N'
|Description||Recombinant Human BMP-7 is a 28.8 kDa homodimeric glycoprotein consisting of two 116 amino acid subunits, which correspond to amino acid residues 316 to 431 of the full-length BMP-7 precursor.|
|Expression cDNA Clone or AA Sequence||MANVAENSSS DQRQACKKHE LYVSFRDLGW QDWIIAPEGY AAYYCE GECA FPLNSYMNAT NHAIVQTLVH FINPETVPKP CCAPTQLNAI S VLYFDDSSN VILKKYRNMV VRACGCH|
|Predicted MW||28.8 kDa|
|Purity||> 98 % by SDS-PAGE & HPLC analysis|
|Buffer||PBS without stabilizers|
|Bioactivity||Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 0.02-0.04 µg/ml.|
|Endotoxin||< 0.1 ng per µg of BMP-7|
|Reconstitution||Restore in PBS to a concentration not lower than 50 µg/ml.
The lyophilized sTIE-2/hFc is soluble in water and most aqueous buffers.
|Preparation||Lyophilized purified protein|
|Summary||This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone, kidney and brown adipose tissue development. Additionally, this protein induces ectopic bone formation and may promote fracture healing in human patients. [provided by RefSeq, Jul 2016]|
|Protein Families||Adult stem cells, Cancer stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Secreted Protein, Stem cell relevant signaling - TGFb/BMP signaling pathway|
|Protein Pathways||Cytokine-cytokine receptor interaction, Hedgehog signaling pathway, TGF-beta signaling pathway|
|TP723047||Purified recombinant protein of Human bone morphogenetic protein 7 (BMP7).||
|AR09130PU-L||Recombinant human BMP-7 protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.||
|LY400647||Transient overexpression lysate of bone morphogenetic protein 7 (BMP7)||
|AR09130PU-N||Recombinant human BMP-7 protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques, after refolding of the isolated inclusion body in renaturation buffer.||
|LC400647||BMP7 HEK293T cell transient overexpression lysate (as WB positive control)||