Recombinant Human Epidermal Growth Factor (EGF).
Product Datasheet for 'AR31147PU-N'
|Description||Recombinant Human Epidermal Growth Factor (EGF).|
|Expression Host||E. coli|
|Expression cDNA Clone or AA Sequence||MNSDSECPLSHDGYCLHDGVCMYIEALDKYACNCVVGYIGERCQYRDLKW WELR.
N-terminal Sequence: MNSDSECPLS
|Predicted MW||6.35 kDa|
|Purity||> 95 % by SDS-PAGE & silver stain|
|Bioactivity||The biological activity was determined by the ability to induce EGF receptor phosphorylation in the A431 tumor cell line [Soler et al, J Chromatography B, 788, 2003] and the induction of proliferation in NHDF cells (Normal Human Dermal Fibroblasts).|
|Reconstitution||A quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml.
This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
|Preparation||Sterile, Lyophilized powder.|
|Summary||This gene encodes a member of the epidermal growth factor superfamily. The encoded preproprotein is proteolytically processed to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding with high affinity to the cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]|
|Protein Families||Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transmembrane|
|Protein Pathways||Bladder cancer, Cytokine-cytokine receptor interaction, Endocytosis, Endometrial cancer, ErbB signaling pathway, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton|
|BIN031||Recombinant Human Epidermal Growth Factor (EGF) containing 1-51 amino acid residues overexpressed in Pichia pastoris.
Single immunospecific band at 5 kDa and may appear as a doublet.
|TP723068||Purified recombinant protein of Human epidermal growth factor (EGF), transcript variant 1.||
|AR50004PU-N||Recombinant EGF was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.||
|TP310817||Recombinant protein of human epidermal growth factor (beta-urogastrone) (EGF)||
|TP723821||Purified recombinant protein of Human epidermal growth factor (EGF), transcript variant 1||
|LC419624||EGF HEK293T cell transient overexpression lysate (as WB positive control)||
|AR50004PU-S||Recombinant EGF was expressed in E.coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.||
|TP720580||Purified recombinant protein of Human epidermal growth factor (EGF), transcript variant 1||
|LY419624||Transient overexpression lysate of epidermal growth factor (beta-urogastrone) (EGF)||
|AR31156PU-N||Recombinant Human EGF.
N-terminal seq: MNSDSECPLS
|PH310817||EGF MS Standard C13 and N15-labeled recombinant protein (NP_001954)||