DHFR / DHFRP1 (1-187, His-tag) Mouse Protein

CAT#: AR09852PU-L

DHFR / DHFRP1 (1-187, His-tag) mouse recombinant protein, 0.5 mg

Product Images

Specifications

Product Data

• Species: Mouse
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF EVYEKKD
• Tag: His-tag
• Predicted MW: 23.8 kDa
• Concentration: lot specific
• Purity: >95%
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2 mM DTT, 0.1M NaCl
• Bioactivity: Specific: > 0.2 units/mg, in which one unit will convert 1.0 umole of 7,8– dihydrofloate and beta - NADPH to 5,6,7,8 - tetrahydrofloate and beta - NADP per min at pH 6.5 at 25°C
  Activity Assay:
  1. Prepare a 1.55 ml assay buffer. The final concentrations are 50 mM potassium phosphate, 0.072 mM dihydrofolic acid, 0.1 mM beta-nicotinamide dinucleotide phosphate and 0.003 % (w/v) bovine serum albumin.
  2. Add 50 ul of recombinant DHFR protein in various concentrations (2 ug, 5 ug) in assay buffer.
  3. Mix by inversion and record the decrease at A340nm for 5 minutes.
• Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
• Preparation: Liquid purified protein
• Protein Description: Recombinant mouse DHFR protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_034179
• Locus ID: 13361
• UniProt ID: P00375, Q544T5
• Cytogenetics: 13 47.64 cM
• Synonyms: 8430436I03Rik; AA607882; AI662710; AW555094
• Summary: Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway (PubMed:25980602). Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (PubMed:25980602). Binds its own mRNA.[UniProtKB/Swiss-Prot Function]