MDH2 (25-338, His-tag) Human Protein

CAT#: AR09617PU-N

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MDH2 (25-338, His-tag) human recombinant protein, 50 µg



USD 330.00


Availability*
2 Weeks

Size
    • 50 ug


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Specifications

Product Data
Description MDH2 (25-338, His-tag) human recombinant protein, 50 µg
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence MGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET KAAVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMICVIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL TALTGRIQEA GTEVVKAKAG AGSATLSMAY AGARFVFSLV DAMNGKEGVV ECSFVKSQET ECTYFSTPLL LGKKGIEKNL GIGKVSSFEE KMISDAIPEL KASIKKGEDF VKTLK
Tag His-tag
Predicted MW 35.2 kDa
Concentration 1.0 mg/ml (determined by Bradford assay)
Purity >95% by SDS - PAGE
Buffer Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris-HCl buffer (pH 7.5) containing 10% Glycerol
Bioactivity Biological: Specific activity is > 30 units/mg, and is defined as the amount of enzyme that cleaves 1 umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH 7.5 at 25°C (see "Protocols").
Preparation Liquid purified protein
Applications Protocol: Activity Assay
1. Prepare assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 100 mM Potassium phosphate, 0.13 mM beta-nicotinamide adenine dinucleotide, reduced form, 0.25 mM oxalacetic acid.
2. Add recombinant MDH protein with various concentrations (0.1ug, 0.5ug, 1ug) in assay buffer.
3. Mix by inversion and equilibrate to 1°C and monitor at A340nm until the value is constant using a spectrophotometer.
4. Record the increase at A340nm for approximately 2 minutes.
Protein Description Recombinant human MDH2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_001269332
Locus ID 4191
Cytogenetics 7q11.23
Synonyms EIEE51; M-MDH; MDH; MGC:3559; MOR1
Summary Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
Protein Families Druggable Genome
Protein Pathways Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways, Pyruvate metabolism

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