Calreticulin (18-417, His-tag)

CAT#: AR09452PU-N

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Recombinant human Calreticulin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.


 Product Datasheet for 'AR09452PU-N'

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USD 400.00


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2 Weeks

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    • 100 ug


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Product Data
Description Recombinant human Calreticulin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence MGSSHHHHHH SSGLVPRGSH MEPAVYFKEQ FLDGDGWTSR WIESKHKSDF GKFVLSSGKF YGDEEKDKGL QTSQDARFYA LSASFEPFSN KGQTLVVQFT VKHEQNIDCG GGYVKLFPNS LDQTDMHGDS EYNIMFGPDI CGPGTKKVHV IFNYKGKNVL INKDIRCKDD EFTHLYTLIV RPDNTYEVKI DNSQVESGSL EDDWDFLPPK KIKDPDASKP EDWDERAKID DPTDSKPEDW DKPEHIPDPD AKKPEDWDEE MDGEWEPPVI QNPEYKGEWK PRQIDNPDYK GTWIHPEIDN PEYSPDPSIY AYDNFGVLGL DLWQVKSGTI FDNFLITNDE AYAEEFGNET WGVTKAAEKQ MKDKQDEEQR LKEEEEDKKR KEEEEAEDKE DDEDKDEDEE DEEDKEEDEE EDVPGQAKDE L
Tag His-tag
Predicted MW 48.7 kDa
Concentration 1.0 mg/ml (determined by Bradford assay)
Purity > 85 % by SDS - PAGE
Buffer 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 0.1 M Nacl, 10% glycerol
Preparation Liquid purified protein
Reference Data
RefSeq NP_004334
Locus ID 811
Cytogenetics 19p13.13
Synonyms cC1qR; CRT; HEL-S-99n; RO; SSA
Summary Calreticulin is a multifunctional protein that acts as a major Ca(2+)-binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes. [provided by RefSeq, Jul 2008]
Protein Families Druggable Genome, Secreted Protein, Transcription Factors
Protein Pathways Antigen processing and presentation

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BOGO Free lysates
68 Mouse Clones