Thioredoxin reductase / TRXB (1-321) Escherichia coli Protein

CAT#: AR09450PU-N

Thioredoxin reductase / TRXB (1-321) e. coli recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Escherichia coli
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGTTKHSKLL ILGSGPAGYT AAVYAARANL QPVLITGMEK GGQLTTTTEV ENWPGDPNDL TGPLLMERMH EHATKFETEI IFDHINKVDL QNRPFRLNGD NGEYTCDALI IATGASARYL GLPSEEAFKG RGVSACATCD GFFYRNQKVA VIGGGNTAVE EALYLSNIAS EVHLIHRRDG FRAEKILIKR LMDKVENGNI ILHTNRTLEE VTGDQMGVTG VRLRDTQNSD NIESLDVAGL FVAIGHSPNT AIFEGQLELE NGYIKVQSGI HGNATQTSIP GVFAAGDVMD HIYRQAITSA GTGCMAALDA ERYLDGLADA K
• Predicted MW: 34.6 kDa
• Concentration: lot specific
• Purity: >90% pure by SDS - PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 10% glycerol
• Bioactivity: Biological: Specific activity is > 10 units/mg, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm.
  Activity Assay
  1. Prepare a 0.7 ml reaction mixture into a suitable container: The final concentrations are 100mM potassium phosphate, 10mM EDTA, 0.2mM beta-NADPH, 0.05% BSA, 0.014% (w/v) thioredoxin, 5mM DTNB, 5ug E.coli TRXB.
  2. Equilibrate to 25°C and monitor the A412nm until the value is constant using a spectrophotometer.
  3. Add 35 ul of 100 mM DTNB into reaction mixture and mix immediately.
  4. Record the increase in A412nm for 2 minutes.
• Preparation: Liquid purified protein
• Protein Description: Recombinant E.coli TRXB protein was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• Summary: TRXB (Thioredoxin reductase) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress. The mammalian Thioredoxin reductase reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide.