AKR7A2 (1-359, His-tag) Human Protein

CAT#: AR09377PU-N

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AKR7A2 (1-359, His-tag) human recombinant protein, 0.1 mg



USD 457.00


Availability*
3 Weeks

Size
    • 100 ug


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Specifications

Product Data
Description AKR7A2 (1-359, His-tag) human recombinant protein, 0.1 mg
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM LSAASRVVSR AAVHCALRSP PPEARALAMS RPPPPRVASV LGTMEMGRRM DAPASAAAVR AFLERGHTEL DTAFMYSDGQ SETILGGLGL GLGGGDCRVK IATKANPWDG KSLKPDSVRS QLETSLKRLQ CPQVDLFYLH APDHGTPVEE TLHACQRLHQ EGKFVELGLS NYASWEVAEI CTLCKSNGWI LPTVYQGMYN ATTRQVETEL FPCLRHFGLR FYAYNPLAGG LLTGKYKYED KDGKQPVGRF FGNSWAETYR NRFWKEHHFE AIALVEKALQ AAYGASAPSV TSAALRWMYH HSQLQGAHGD AVILGMSSLE QLEQNLAATE EGPLEPAVVD AFNQAWHLVA HECPNYFR
Tag His-tag
Concentration lot specific
Purity >90% by SDS - PAGE
Buffer Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 20% glycerol
Bioactivity Biological: Specific activity is approximately 0.25-0.3 units/mg protein.
Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C.




Activity Assay
1. Prepare a 1.0 ml reaction mix into a suitable container : The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH.
2. Add 50 ul of recombinant AKR7A2 solution with various concentrations (2.5ug, 5ug, 10ug) in 750 ul reaction buffer.
3. Mix by inversion and incubate at 25°C for 2.5 minutes.
4. Add 200 ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion.
5. Record the increase in A340nm for 3 minutes.
Preparation Liquid purified protein
Protein Description Recombinant AKR7A2 protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_001307908
Locus ID 8574
UniProt ID B4DZX4
Cytogenetics 1p36.13
Synonyms AFAR; AFAR1; AFB1-AR1; AKR7
Summary The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
Protein Families Druggable Genome
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