AKR7A2 (1-359, His-tag) Human Protein

CAT#: AR09377PU-N

AKR7A2 (1-359, His-tag) human recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM LSAASRVVSR AAVHCALRSP PPEARALAMS RPPPPRVASV LGTMEMGRRM DAPASAAAVR AFLERGHTEL DTAFMYSDGQ SETILGGLGL GLGGGDCRVK IATKANPWDG KSLKPDSVRS QLETSLKRLQ CPQVDLFYLH APDHGTPVEE TLHACQRLHQ EGKFVELGLS NYASWEVAEI CTLCKSNGWI LPTVYQGMYN ATTRQVETEL FPCLRHFGLR FYAYNPLAGG LLTGKYKYED KDGKQPVGRF FGNSWAETYR NRFWKEHHFE AIALVEKALQ AAYGASAPSV TSAALRWMYH HSQLQGAHGD AVILGMSSLE QLEQNLAATE EGPLEPAVVD AFNQAWHLVA HECPNYFR
• Tag: His-tag
• Concentration: lot specific
• Purity: >90% by SDS - PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 20% glycerol
• Bioactivity: Biological: Specific activity is approximately 0.25-0.3 units/mg protein.
  Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C.
  
  
  
  
  Activity Assay
  1. Prepare a 1.0 ml reaction mix into a suitable container : The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH.
  2. Add 50 ul of recombinant AKR7A2 solution with various concentrations (2.5ug, 5ug, 10ug) in 750 ul reaction buffer.
  3. Mix by inversion and incubate at 25°C for 2.5 minutes.
  4. Add 200 ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion.
  5. Record the increase in A340nm for 3 minutes.
• Preparation: Liquid purified protein
• Protein Description: Recombinant AKR7A2 protein, fused to His-tag, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001307908
• Locus ID: 8574
• UniProt ID: B4DZX4
• Cytogenetics: 1p36.13
• Synonyms: AFAR; AFAR1; AFB1-AR1; AKR7
• Summary: The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2016]
• Protein Families: Druggable Genome