Cytokeratin 18 Human Protein
Cytokeratin 18 human recombinant protein, 0.25 mg
|Description||Cytokeratin 18 human recombinant protein, 0.25 mg|
|Purity||>95% pure (determined by SDS gelelectrophoresis)|
|Buffer||Presentation State: Purified
State: Lyophilized purified protein
Buffer System: 30mM Tris/HCI pH 8, 9.5M Urea, 2mM DTT, 2mM EDTA, 10 mM Methylammonium Chloride
|Reconstitution||Restore with 175 µl distilled water (final volume 250 µl für AR09362PU-N).
Restore with 70 µl distilled water (final volume 100 µl für AR09362PU-S).
|Preparation||Lyophilized purified protein|
|Applications||Protein standard in 1D and 2D SDS gelelectrophoresis.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above).
Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4).
For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g.Dulbecco's PBS).
|Protein Description||Recombinant Human keratin K18 Standard (formerly also designated Cytokeratin 18).|
|Note||Isoelectric Point: pI 5.7|
|Storage||Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Synonyms||CK-18; CYK18; K18|
|Summary||KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]|
|Protein Pathways||Pathogenic Escherichia coli infection|
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