Alpha-enolase / ENO1 (1-434) Human Protein

CAT#: AR09287PU-N

Alpha-enolase / ENO1 (1-434) human recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK
• Predicted MW: 47.1 kDa
• Concentration: lot specific
• Purity: >95% by SDS-PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM MgSO₄, 10% Glycerol
• Bioactivity: Specific: > 20,000 pmol/min/µg, and was obtained by measuring the decrease of NAD in absorbance at 340nm resulting from NADH at pH 6.5 at 37°C.
  
  Activity Assay
  1. Prepare 180 µl assay buffer into a suitable container: The concentrations are 80 mM Triethanolamine (pH 6.5), 0.3 mM 2-phosphorglycerate, 0.2 mM beta-NADH, 25 mM magnesium sulfate, 100 mM potassium chloride, 1.2 mM ADP, 3.5 unit pyruvate kinase, 5 unit L-lactic dehydrogenas.
  2. Equilibrate to 37°C and monitor at A340nm until the value is constant using a spectrophotometer.
  3. Add 20 µl of recombinant a-enolase protein 10 µg/ml in assay buffer.
  4. Record the decrease in A340nm for 5 minutes at 37°C
• Endotoxin: < 1.0 EU per 1 µg of protein (determined by LAL method)
• Preparation: Liquid purified protein
• Protein Description: Recombinant Human Alpha-enolase was expressed in E.coli and purified by using conventional chromatography.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001188412
• Locus ID: 2023
• UniProt ID: P06733
• Cytogenetics: 1p36.23
• Synonyms: ENO1L1; HEL-S-17; MPB1; NNE; PPH
• Summary: This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011]
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation