Peroxiredoxin-6 / PRDX6 (1-224, His-tag) Human Protein

CAT#: AR09237PU-N

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Peroxiredoxin-6 / PRDX6 (1-224, His-tag) human recombinant protein, 0.1 mg



USD 330.00


Availability*
2 Weeks

Size
    • 100 ug


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Specifications

Product Data
Description Peroxiredoxin-6 / PRDX6 (1-224, His-tag) human recombinant protein, 0.1 mg
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence MGSSHHHHHH SSGLVPRGSH MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQP
Tag His-tag
Predicted MW 27.1 kDa
Concentration lot specific
Purity >95% by SDS - PAGE
Buffer Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris-HCl buffer (pH 8.0) containing 20% Glycerol
Bioactivity Specific: Approximately 95-120 pmole/min/µg.
Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX6 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25°C for 1 minute.
Preparation Liquid purified protein
Applications Protocol: Activity Assay:
1. Prepare a 50ul reaction mix into a suitable container :
The final concentrations are 1mM DTT, 0.03X PBS, 0.5% glycerol.
2. Add 5 ul of recombinant PRDX6 solution with various concentrations (0.25ug, 0.5ug) in 45 ul reaction buffer.
3. Incubate at 25°C for 2 minutes.
4. Add 5ul of 5 mM H2O2 as a substrate and incubate the mixture for 20 min.
5. Add 20ul of 26 % trichloroacetic acid (TCA) to stop the reaction.
6. Add 30ul of Formation solution (10mM Ferrous ammonium sulfate (Fe(II)(NH4)2(SO4)2), 2.5M KSCN)
7. Record the increase in A475nm.
Protein Description Recombinant human peroxiredoxin 6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_004896
Locus ID 9588
Cytogenetics 1q25.1
Synonyms 1-Cys; aiPLA2; AOP2; HEL-S-128m; NSGPx; p29; PRX
Summary The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008]
Protein Families Druggable Genome
Protein Pathways Metabolic pathways, Methane metabolism, Phenylalanine metabolism
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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