Phosphoglycerate kinase 1 (PGK1) (24-91, His-tag) Human Protein
Phosphoglycerate kinase 1 (PGK1) (24-91, His-tag) human recombinant protein, 0.1 mg
|Description||Phosphoglycerate kinase 1 (PGK1) (24-91, His-tag) human recombinant protein, 0.1 mg|
|Expression Host||E. coli|
|Expression cDNA Clone or AA Sequence||MGSSHHHHHH SSGLVPRGSH MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV GPEVEKACAN PAAGSVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN MEIGTSLFDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ ATVASGIPAG WMGLDCGPES SKKYAEAVTR AKQIVWNGPV GVFEWEAFAR GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VDALSNI|
|Predicted MW||46.8 kDa|
|Purity||>95% by SDS - PAGE|
|Buffer||Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris (pH 8.0), containing 10% Glycerol, 1 mM DTT
|Endotoxin||< 1.0 EU per 1 µg of protein (determined by LAL method )|
|Preparation||Liquid purified protein|
|Protein Description||Recombinant PGK1 protein, fused to His-Tag, was expressed in E.coli and purified by using conventional chromatography techniques.|
|Storage||Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Synonyms||HEL-S-68p; MIG10; PGKA|
|Summary||The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]|
|Protein Families||Druggable Genome|
|Protein Pathways||Glycolysis / Gluconeogenesis, Metabolic pathways|
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