AKR1B10 (1-316)

CAT#: AR09195PU-L

Reviews ()
Write a review

Recombinant Human AKR1B10 protein was expressed in E.coli and purified by using conventional chromatography.


Product Datasheet for 'AR09195PU-L'


 Cited in 1 publication.

  View other "AKR1B10" proteins (5)

USD 845.00


Availability*
2 Weeks

Size
    • 500 ug


Product images

Specifications

Product Data
Description Recombinant Human AKR1B10 protein was expressed in E.coli and purified by using conventional chromatography.
Species Human
Expression Host E. coli
Concentration 1 mg/ml (determined by Bradford assay)
Purity > 95 % pure by SDS-PAGE
Buffer 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol
Bioactivity Approximately 0.32-0.4 units/mg protein.
Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C.
Activity Assay
1. Prepare a 1.0 ml reaction mix into a suitable container: The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH.
2. Add 50 ul of recombinant AKR1B10 solution with various concentrations (2.5ug, 5ug, 10ug) in 750 ul reaction buffer.
3. Mix by inversion and incubate at 25°C for 2.5 minutes.
4. Add 200 ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion.
5. Record the increase in A340nm for 3 minutes.
Preparation Liquid purified protein
Reference Data
RefSeq NP_064695
Locus ID 57016
Cytogenetics 7q33
Synonyms AKR1B11; AKR1B12; ALDRLn; ARL-1; ARL1; HIS; HSI
Summary This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq, Jul 2008]
Protein Families Druggable Genome
Protein Pathways Butanoate metabolism, Fructose and mannose metabolism, Linoleic acid metabolism, Metabolic pathways

Citations (1)

Documents

Other Versions

Other products for "AKR1B10"
Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
68 Mouse Clones