Recombinant Human AKR1B10 protein was expressed in E.coli and purified by using conventional chromatography.
|Description||Recombinant Human AKR1B10 protein was expressed in E.coli and purified by using conventional chromatography.|
|Expression Host||E. coli|
|Concentration||1 mg/ml (determined by Bradford assay)|
|Purity||> 95 % pure by SDS-PAGE|
|Buffer||20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol|
|Bioactivity||Approximately 0.32-0.4 units/mg protein.
Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25°C.
1. Prepare a 1.0 ml reaction mix into a suitable container: The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH.
2. Add 50 ul of recombinant AKR1B10 solution with various concentrations (2.5ug, 5ug, 10ug) in 750 ul reaction buffer.
3. Mix by inversion and incubate at 25°C for 2.5 minutes.
4. Add 200 ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion.
5. Record the increase in A340nm for 3 minutes.
|Preparation||Liquid purified protein|
|Synonyms||AKR1B11; AKR1B12; ALDRLn; ARL-1; ARL1; HIS; HSI|
|Summary||This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq, Jul 2008]|
|Protein Families||Druggable Genome|
|Protein Pathways||Butanoate metabolism, Fructose and mannose metabolism, Linoleic acid metabolism, Metabolic pathways|
|The use of this Proteins has been cited in the following citations:|
Identification of novel aldose reductase inhibitors based on carboxymethylated mercaptotriazinoindole scaffold
,Stefek M, Soltesova Prnova M, Majekova M, Rechlin C, Heine A, Klebe G,
J. Med. Chem. 2016
,PubMed ID 25695864
|PH303177||AKR1B10 MS Standard C13 and N15-labeled recombinant protein (NP_064695)||
|AR09195PU-N||Recombinant Human AKR1B10 protein was expressed in E.coli and purified by using conventional chromatography.||
|LC402772||AKR1B10 HEK293T cell transient overexpression lysate (as WB positive control)||
|LY402772||Transient overexpression lysate of aldo-keto reductase family 1, member B10 (aldose reductase) (AKR1B10)||
|TP303177||Recombinant protein of human aldo-keto reductase family 1, member B10 (aldose reductase) (AKR1B10)||