Interleukin-15 / IL15 (49-162, His-tag) Mouse Protein

CAT#: AR09175PU-L

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Interleukin-15 / IL15 (49-162, His-tag) mouse recombinant protein, 0.25 mg


Product Datasheet for 'AR09175PU-L'


USD 845.00


Availability*
2 Weeks

Size
    • 250 ug


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Specifications

Product Data
Description Interleukin-15 / IL15 (49-162, His-tag) mouse recombinant protein, 0.25 mg
Species Mouse
Expression Host E. coli
Expression cDNA Clone or AA Sequence MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMNW IDVRYDLEKI ESLIQSIHID TTLYTDSDFH PSCKVTAMNC FLLELQVILH EYSNMTLNET VRNVLYLANS TLSSNKNVAE SGCKECEELE EKTFTEFLQS FIRIVQMFIN TS
Tag His-tag
Predicted MW 17.6 kDa
Concentration 0.5 mg/ml (determined by Bradford assay)
Purity >90% by SDS - PAGE
Buffer Presentation State: Purified
State: Liquid purified protein
Buffer System: PBS, pH 7.4, containing 10% glycerol
Bioactivity Biological: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic
T cells. The ED50 for this effect is < 1 ng/ml.
Activity Assay
1. Cell line: CTLL2 (mouse cytotoxic T cell)
2. Maintenance Condition: 10% FBS RPMI 1640 with hIL2
3. Assay Medium: 10% FBS RPMI 1640 without hIL2
4. Cell Density: 2 x 10e4 cells/well (96 well plate, final volume 100ul)
5. Starvation : 24hr, Assay medium
6. Incubation Time : 24hr (after sample treatment)
7. Concentration Range: 0.039 ng/ml - 40 ng/ml
8. Detection method : MTT assay
Endotoxin < 1.0 EU per 1 µg of protein (determined by LAL method )
Preparation Liquid purified protein
Protein Description Recombinant mouse IL-15, His-tagged, was expressed in E.coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_001241676
Locus ID 16168
Cytogenetics 8 39.33 cM
Synonyms AI503618; IL-15
Summary This gene encodes a a pleiotropic cytokine of the interleukin family of proteins that plays important roles in the innate and adaptive cell homeostasis, as well as peripheral immune function. The encoded protein undergoes proteolytic processing to generate a mature cytokine that stimulates the proliferation of natural killer cells. The transgenic mice overexpressing the encoded protein exhibit an increase in the number of memory CD8+ T cells in a naive state and enhanced protection against bacterial infections. Mice lacking the encoded protein exhibit impaired protection against a strain of attenuated Mycobacterium. [provided by RefSeq, Aug 2016]

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