Interleukin-15 / IL15 (49-162, His-tag)

CAT#: AR09175PU-L

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Recombinant mouse IL-15, His-tagged, was expressed in E.coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.


 Product Datasheet for 'AR09175PU-L'

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USD 820.00


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2 Weeks

Size
    • 250 ug


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Product Data
Description Recombinant mouse IL-15, His-tagged, was expressed in E.coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.
Species Mouse
Expression Host E. coli
Tag His-tag
Predicted MW 17.6 kDa
Concentration 0.5 mg/ml (determined by Bradford assay)
Purity > 90 % by SDS - PAGE
Buffer PBS, pH 7.4, containing 10% glycerol
Bioactivity Measured in a cell proliferation assay using CTLL2 mouse cytotoxic
T cells. The ED50 for this effect is < 1 ng/ml.
Activity Assay
1.\tCell line: CTLL2 (mouse cytotoxic T cell)
2.\tMaintenance Condition: 10% FBS RPMI 1640 with hIL2
3.\tAssay Medium: 10% FBS RPMI 1640 without hIL2
4.\tCell Density: 2 x 10e4 cells/well (96 well plate, final volume 100ul)
5.\tStarvation : 24hr, Assay medium
6.\tIncubation Time : 24hr (after sample treatment)
7.\tConcentration Range: 0.039 ng/ml - 40 ng/ml
8.\tDetection method : MTT assay
Endotoxin < 1.0 EU per 1 µg of protein (determined by LAL method)
Preparation Liquid purified protein
Reference Data
RefSeq NP_001241676
Locus ID 16168
Synonyms AI503618; IL-15
Summary This gene encodes a a pleiotropic cytokine of the interleukin family of proteins that plays important roles in the innate and adaptive cell homeostasis, as well as peripheral immune function. The encoded protein undergoes proteolytic processing to generate a mature cytokine that stimulates the proliferation of natural killer cells. The transgenic mice overexpressing the encoded protein exhibit an increase in the number of memory CD8+ T cells in a naive state and enhanced protection against bacterial infections. Mice lacking the encoded protein exhibit impaired protection against a strain of attenuated Mycobacterium. [provided by RefSeq, Aug 2016]

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68 Mouse Clones