PRMT1 (Isoform 3) (1-353, His-MBP-tagged) Human Protein

CAT#: AR09076PU-N

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PRMT1 (Isoform 3) (1-353, His-MBP-tagged) human recombinant protein, 0.1 mg



USD 260.00


Availability*
2 Weeks

Size
    • 100 ug


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Specifications

Product Data
Description PRMT1 (Isoform 3) (1-353, His-MBP-tagged) human recombinant protein, 0.1 mg
Species Human
Expression Host E. coli
Expression cDNA Clone or AA Sequence MHHHHHHMKI EEGKLVIWIN GDKGYNGLAE VGKKFEKDTG IKVTVEHPDK LEEKFPQVAA TGDGPDIIFW AHDRFGGYAQ SGLLAEITPD KAFQDKLYPF TWDAVRYNGK LIAYPIAVEA LSLIYNKDLL PNPPKTWEEI PALDKELKAK GKSALMFNLQ EPYFTWPLIA ADGGYAFKYE NGKYDIKDVG VDNAGAKAGL TFLVDLIKNK HMNADTDYSI AEAAFNKGET AMTINGPWAW SNIDTSKVNY GVTVLPTFKG QPSKPFVGVL SAGINAASPN KELAKEFLEN YLLTDEGLEA VNKDKPLGAV ALKSYEEELA KDPRIAATME NAQKGEIMPN IPQMSAFWYA VRTAVINAAS GRQTVDEALK DAQTNSSSNN NNNNNNNNLG IEGRGSHMAA AEAANCIMEV SCGQAESSEK PNAEDMTSKD YYFDSYAHFG IHEEMLKDEV RTLTYRNSMF HNRHLFKDKV VLDVGSGTGI LCMFAAKAGA RKVIGIECSS ISDYAVKIVK ANKLDHVVTI IKGKVEEVEL PVEKVDIIIS EWMGYCLFYE SMLNTVLYAR DKWLAPDGLI FPDRATLYVT AIEDRQYKDY KIHWWENVYG FDMSCIKDVA IKEPLVDVVD PKQLVTNACL IKEVDIYTVK VEDLTFTSPF CLQVKRNDYV HALVAYFNIE FTRCHKRTGF STSPESPYTH WKQTVFYMED YLTVKTGEEI FGTIGMRPNA KNNRDLDFTI DLDFKGQLCE LSCSTDYRMR
Tag His-MBP-tag
Concentration 1.0 mg/ml (determined by Bradford assay)
Purity ≥90 by SDS PAGE
Buffer Presentation State: Purified
State: Liquid purified protein
Buffer System: 40 mM Tris pH 8.0, 100 mM NaCl, 4 mM MgCl2, 2 mM DTT, 40% glycerol
Bioactivity Specific: - Unit Definition: One unit will transfer 1pmol of methyl group to synthetic peptide of histone H4 for 10 minutes at 37°C.
Preparation Liquid purified protein
Protein Description Recombinant human PRMT1, fused to His-MBP tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Storage Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Reference Data
RefSeq NP_001193971
Locus ID 3276
Cytogenetics 19q13.33
Synonyms ANM1; HCP1; HRMT1L2; IR1B4
Summary This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

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