Hexokinase-2 (1-917, His-tag)

CAT#: AR09039PU-L

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Recombinant human Hexokinase 2, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.


 Product Datasheet for 'AR09039PU-L'

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USD 1,620.00


Availability*
2 Weeks

Size
    • 500 ug


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Product Data
Description Recombinant human Hexokinase 2, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Species Human
Expression Host E. coli
Tag His-tag
Predicted MW 104.1 kDa
Concentration 1.0 mg/ml determined by Bradford assay
Purity > 90 % by SDS PAGE
Buffer 20 mM Tris-HCl pH 8.0, 10% glycerol
Bioactivity 3-4 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. In the coupled mode, one unit will produce 1.0 umole of NADPH per minute as glucose is phosphorylated by ATP at pH 7.4 at 30°C.
Preparation Liquid purified protein
Reference Data
RefSeq NP_000180
Locus ID 3099
Cytogenetics 2p12
Synonyms HKII; HXK2
Summary Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells. [provided by RefSeq, Apr 2009]
Protein Families Druggable Genome
Protein Pathways Amino sugar and nucleotide sugar metabolism, Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, Starch and sucrose metabolism, Type II diabetes mellitus

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68 Mouse Clones