GAPDH (1-335) Human Protein

CAT#: AR09029PU-N

GAPDH (1-335) human recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGKVKVGVNG FGRIGRLVTR AAFNSGKVDI VAINDPFIDL NYMVYMFQYD STHGKFHGTV KAENGKLVIN GNPITIFQER DPSKIKWGDA GAEYVVESTG VFTTMEKAGA HLQGGAKRVI ISAPSADAPM FVMGVNHEKY DNSLKIISNA SCTTNCLAPL AKVIHDNFGI VEGLMTTVHA ITATQKTVDG PSGKLWRDGR GALQNIIPAS TGAAKAVGKV IPELNGKLTG MAFRVPTANV SVVDLTCRLE KPAKYDDIKK VVKQASEGPL KGILGYTEHQ VVSSDFNSDT HSSTFDAGAG IALNDHFVKL ISWYDNEFGY SNRVVDLMAH MASKE
• Predicted MW: 36 kDa
• Concentration: lot specific
• Purity: >95% pure by SDS-PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris pH 8.0, 1 mM EDTA, 1 mM DTT, 20% glycerol
• Preparation: Liquid purified protein
• Protein Description: Recombinant GAPDH protein was expressed in E.coli and purified by using conventional chromatography techniques.
• Note: NCBI Accession No.: NP_002037
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C to -80°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001243728
• Locus ID: 2597
• UniProt ID: P04406
• Cytogenetics: 12p13.31
• Synonyms: G3PD; GAPD; HEL-S-162eP
• Summary: This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]
• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways