CD26 / DPP4 (39-766, His-tag) Human Protein
CD26 / DPP4 (39-766, His-tag) human recombinant protein, 100 µg
|Description||CD26 / DPP4 (39-766, His-tag) human recombinant protein, 100 µg|
|Expression Host||Hi-5 insect|
|Expression cDNA Clone or AA Sequence||ADP-R KTYTLTDYLK NTYRLKLYSL RWISDHEYLY KQENNILVFN AEYGNSSVFL ENSTFDEFGH SINDYSISPD GQFILLEYNY VKQWRHSYTA SYDIYDLNKR QLITEERIPN NTQWVTWSPV GHKLAYVWNN DIYVKIEPNL PSYRITWTGK EDIIYNGITD WVYEEEVFSA YSALWWSPNG TFLAYAQFND TEVPLIEYSF YSDESLQYPK TVRVPYPKAG AVNPTVKFFV VNTDSLSSVT NATSIQITAP ASMLIGDHYL CDVTWATQER ISLQWLRRIQ NYSVMDICDY DESSGRWNCL VARQHIEMST TGWVGRFRPS EPHFTLDGNS FYKIISNEEG YRHICYFQID KKDCTFITKG TWEVIGIEAL TSDYLYYISN EYKGMPGGRN LYKIQLSDYT KVTCLSCELN PERCQYYSVS FSKEAKYYQL RCSGPGLPLY TLHSSVNDKG LRVLEDNSAL DKMLQNVQMP SKKLDFIILN ETKFWYQMIL PPHFDKSKKY PLLLDVYAGP CSQKADTVFR LNWATYLAST ENIIVASFDG RGSGYQGDKI MHAINRRLGT FEVEDQIEAA RQFSKMGFVD NKRIAIWGWS YGGYVTSMVL GSGSGVFKCG IAVAPVSRWE YYDSVYTERY MGLPTPEDNL DHYRNSTVMS RAENFKQVEY LLIHGTADDN VHFQQSAQIS KALVDVGVDF QAMWYTDEDH GIASSTAHQH IYTHMSHFIK QCFSLP-SGRLVPRGSHHHHHH|
|Predicted MW||86.4 kDa|
|Purity||>95% by SDS PAGE|
|Buffer||Presentation State: Purified
State: Liquid purified protein
Buffer System: 20 mM Tris-HCl pH 8.0, 100 mM NaCl, 1 mM EDTA, 10% glycerol
|Bioactivity||Biological: Approximately > 50 Unit/mg.
One unit will hydrolyze 1 micromole of p-nitroaniline per minute at pH 8.0 at 37°C using 1mM of Gly-Pro p-nitroanilde as a substrate.
Assay procedure and results:
- Reaction buffer: 20mM Tris pH 8.0, 0.1M NaCl, 1mM EDTA
- Total reaction volume: 100 ul
- Reaction temperature: 37°C
1. Add the reaction buffer to each well
2. Add the 10 ul of 10 mM substrate (Gly-Pro p-nitroanilide) to each well
3. Add the enzyme (DPP-4) diluent to each well
4. Incubate the 96 well plate at 37°C.
5. Read the optical density at 405 nm.
(see "Pictures" below)
|Endotoxin||< 1.0 EU per 1 microgram of protein (determined by LAL method)|
|Preparation||Liquid purified protein|
|Protein Description||Recombinant human DPP4 protein was expressed with c-terminal His-tag in high-5 cells using baculovirus expression system and purified by using conventional chromatography techniques.|
|Storage||Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
Avoid repeated freezing and thawing.
|Stability||Shelf life: one year from despatch.|
|Synonyms||ADABP; ADCP2; CD26; DPPIV; TP103|
|Summary||The DPP4 gene encodes dipeptidyl peptidase 4, which is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic type II transmembrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. Dipeptidyl peptidase 4 is highly involved in glucose and insulin metabolism, as well as in immune regulation. This protein was shown to be a functional receptor for Middle East respiratory syndrome coronavirus (MERS-CoV), and protein modeling suggests that it may play a similar role with SARS-CoV-2, the virus responsible for COVID-19. [provided by RefSeq, Apr 2020]|
|Protein Families||Druggable Genome, Protease, Secreted Protein, Transmembrane|