Cyclophilin A (1-165, His-tag) Human Protein

CAT#: AR09015PU-N

Cyclophilin A (1-165, His-tag) human recombinant protein, 0.1 mg

Product Images

Specifications

Product Data

• Species: Human
• Expression Host: E. coli
• Expression cDNA Clone or AA Sequence:
  Protein Sequence

  MGSSHHHHHH SSGLVPRGSH MVNPTVFFDI AVDGEPLGRV SFELFADKVP KTAENFRALS TGEKGFGYKG SCFHRIIPGF MCQGGDFTRH NGTGGKSIYG EKFEDENFIL KHTGPGILSM ANAGPNTNGS QFFICTAKTE WLDGKHVVFG KVKEGMNIVE AMERFGSRNG KTSKKITIAD CGQLE
• Tag: His-tag
• Predicted MW: 20 kDa
• Concentration: lot specific
• Purity: >95% by SDS PAGE
• Presentation: Purified
• Buffer: Presentation State: Purified
  State: Liquid purified protein
  Buffer System: 20 mM Tris-HCl (pH 8.0) containing 20 mM NaCl, 0.5 mM DTT, 10% glycerol
• Bioactivity: Specific: > 270 nmoles/min/mg, defined as the amount of enzyme that cleaves
  1 umole of suc-AAPF-pNA per minute at 25°C in Tris-HCl pH 8.0 using chymotrypsin.
• Endotoxin: < 1.0 EU per 1 μg of protein (determined by LAL method)
• Preparation: Liquid purified protein
• Applications: Protocol: Activity Assay
  1. Prepare 170 ul assay buffer into a suitable container and pre-chill on ice before use:
  The final concentrations are 200 mM Tris-HCl, pH 8.0, and 20nM chymotrypsin.
  2. Add 10 ul of recombinant Cyclophilin A protein with 1 ug in assay buffer.
  3. Mix by inversion and equilibrate to 1°C and monitor the A405nm until the value is constant using a spectrophotometer.
  4. Add 20 ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)
  5. Record the increase in A405 nm for 30 minutes at 25°C.
• Protein Description: Recombinant Human PPIA, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C or -70°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.

Reference Data

• RefSeq: NP_001287910
• Locus ID: 5478
• UniProt ID: P62937, Q567Q0
• Cytogenetics: 7p13
• Synonyms: CYPA; CYPH; HEL-S-69p
• Summary: This gene encodes a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. The encoded protein is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. The protein can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported. [provided by RefSeq, Jul 2008]