GST-Tag (1-218) Schistosoma japonicum Protein

SKU
AR03011PU-L
Recombinant S. Japonicum Glutathione S-transferase/GST protein
  $1,135.00
3 Weeks*
Bulk/Customize
Specifications
Specifications
Product Data
Species Schistosoma japonicum
Expression Host E. coli
Expression cDNA Clone or AA Sequence
Protein Sequence
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR
Predicted MW 26 kDa (224aa)
Concentration 1mg/ml (determined by Bradford assay)
Purity > 95% by SDS-PAGE
Presentation State Purified
Buffer Liquid in. Phosphate-Buffered Saline (pH 7.4)
Bioactivity Specific activity is > 20unit/mg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
Preparation Liquid purified protein
Recommended Dilution SDS-PAGE, Enzyme Activity
Protein Description Recombinant GST was expressed in E.coli and purified by conventional chromatography techniques.
Storage Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80C. Avoid repeated freezing and thawing cycles.
Stability Shelf life: one year from despatch.
Shipping Blue Ice
Reference Data
RefSeq AAB59203.1
UniProt ID P08515
Synonyms Glutathione S-transferase class-mu 26 kDa isozyme, GST 26, Sj26 antigen, SjGST
Summary Glutathione S-transferase (GST) represents a major group of detoxification enzymes. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide range of compounds, including carcinogens, therapeutic drugs, and products of oxidative stress. As well as its enzymatic activities, GST may also bind toxins and function as transport protein. Because of this, an early term for GSTs was ligandin. Glutathione S-transferase was originally separated from Schistosoma japonicum but currently isolated from recombinant E. coli source. Recombinant GST was expressed in E. coli and purified by conventional chromatography techniques.
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