Alpha-amylase 1 / AMY1
Amylase alpha, Human Saliva. Contaminants: Lipase: < 0.001% Proteases: < 0.001% Ammonia: < 0.0121 Umole/mg
Product Datasheet for 'AR00016PU-N'
|Description||Amylase alpha, Human Saliva. Contaminants: Lipase: < 0.001% Proteases: < 0.001% Ammonia: < 0.0121 Umole/mg|
|Concentration||0.18 mg Protein/mg powder (Coomassie)|
|Purity||> 90 % by SDS-PAGE|
|Buffer||150mM Sodium Chloride, 20mM Tris, 1mM Calcium Chloride, pH 7.2 without preservatives.|
|Bioactivity||106.2 Units/mg powder at 37°C.
590 Units/mg Protein.One unit will catalyze the hydrolysis of one micromole of malto pentaose, which through coupled reactions results in the formation of 5 micromoles of glucose-6-phosphate per minute at 37°C. Measured at 340nm as an equimolar amount of NADH produced by a coupled reaction.
|Reconstitution||Restore with Tris saline or other buffer to desired concentration.|
|Preparation||Lyophilized purified protein|
|Summary||Amylases are secreted proteins that hydrolyze 1,4-alpha-glucoside bonds in oligosaccharides and polysaccharides, and thus catalyze the first step in digestion of dietary starch and glycogen. The human genome has a cluster of several amylase genes that are expressed at high levels in either salivary gland or pancreas. This gene encodes an amylase isoenzyme produced by the salivary gland. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]|
|Protein Families||ES Cell Differentiation/IPS, Secreted Protein|
|Protein Pathways||Metabolic pathways, Starch and sucrose metabolism|
|LY418295||Transient overexpression lysate of amylase, alpha 1A (salivary) (AMY1A), transcript variant 1||
|LC423398||AMY1A HEK293T cell transient overexpression lysate (as WB positive control)||
|PH317403||AMY1A MS Standard C13 and N15-labeled recombinant protein (NP_004029)||
|TP317403||Recombinant protein of human amylase, alpha 1A (salivary) (AMY1A), transcript variant 1||
|LC418295||AMY1A HEK293T cell transient overexpression lysate (as WB positive control)||
|LY423398||Transient overexpression lysate of amylase, alpha 1A (salivary) (AMY1A), transcript variant 2||