SMAD5 (NM_001001420) Human Over-expression Lysate
Transient overexpression lysate of SMAD family member 5 (SMAD5), transcript variant 3
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|Description||Transient overexpression lysate of SMAD family member 5 (SMAD5), transcript variant 3|
|Expression cDNA Clone or AA Sequence||TrueORF Clone RC224544|
|Detection Antibodies||Anti-DDK Antibody (TA50011-100)|
|Accession Number||NM_001001420, NP_001001420|
|Other Names||SMAD5, DWFC; JV5-1; MADH5|
|Predicted MW||52.1 kDa|
|Components||1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg empty vector transfected control cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT)
|Storage||The lysate is shipped with dry ice. Upon receiving, store the sample at -20ºC. Lysate samples are stable for 12 months from date of receipt when stored at -20ºC. Avoid repeated freeze-thaw cycles.
Lysate samples can be diluted with 2xSDS Sample Buffer provided. After dilution, the protein sample should be aliquoted and stored at -20ºC for long term storage. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
|Preparation||HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). Cell lysates were aliquoted and stored at -20ºC before shipping.|
|Cytogenetics||DWFC; JV5-1; MADH5|
|Protein Families||Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, ES Cell Differentiation/IPS, Cancer stem cells, Transcription Factors, Druggable Genome|
|Protein Pathways||TGF-beta signaling pathway|