S100 alpha 6 (S100A6) (NM_014624) Human Over-expression Lysate

CAT#: LC415155

Reviews ()
Write a review

S100A6 HEK293T cell transient overexpression lysate (as WB positive control)


Product Datasheet for 'LC415155'


  View other "S100A6" proteins (3)

USD 99.00


Availability*
In Stock

Size
    • 20 ug


Product images

Specifications

Product Data
Description S100A6 HEK293T cell transient overexpression lysate (as WB positive control)
Species Human
Expression Host HEK293T
Expression cDNA Clone or AA Sequence TrueORF Clone RC203951
Tag C-Myc/DDK
Detection Antibodies Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100)
Accession Number NM_014624, NP_055439
Other Names 2A9; 5B10; CABP; CACY; PRA; S10A6
Predicted MW 10.2 kDa
Components 1 vial of 20 ug lyophilized gene specific transient over-expression cell lysate
Storage The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20°C. Lysate samples can be reconstituted with SDS Sample Buffer. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples are stable for 12 months from date of receipt when stored at -20°C.
Preparation HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.
Reference Data
RefSeq NP_055439
Locus ID 6277
Cytogenetics 1q21.3

Other Versions

Other products for "S100A6"
Frequently bought together (3)
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

USD 310.00


S100A6 mouse monoclonal antibody, clone OTI5A10 (formerly 5A10)
    • 100 ul

USD 379.00


beta Actin Mouse Monoclonal Antibody, Clone OTI1, Loading Control
    • 30 ul

USD 159.00

Customer Reviews 
Loading...
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
68 Mouse Clones
Molbio tools